This is the home page for the Principles of Metabolism PhD course at KI, march 2017.
Schedule and location
The course will run half-time over two weeks, March 20—31. The intention is to have a slower pace so as to give a bit more time to absorb new concepts and also allow time to maintain ongoing experiments, etc during the course. There will be 6 sessions in total (1 introduction).
All seminars are at Retzius väg 13, Karolinska Campus Solna. See this map.
Video lectures (YouTube) and handouts (PDF) are available below.
Lecture 16. One-carbon units and methylation. Video PDF
Databases and websites
Problem collections for seminars
Seminar 1: Energy and material; oxidation and reduction; carbohydrates. PDF
Seminar 2: Isotope tracing; The TCA cycle. PDF
Seminar 3: Energetics, Respiration; Enzyme classes; Amino acids. PDF
Seminar 4: Measuring metabolism; lipids; nucleotides. PDF
Seminar 5: Compartmentalization and transport. PDF
We will use Stryer’s Biochemistry as a reference text, complementing online materials. The below sections are most relevant to the course; you may want to skim through these sections early on. We will also cover this material in lectures and classroom sessions, but from a somewhat different angle.
- Chapter 8, Enzymes: Basic concepts and kinetics. pp. 205 – 212.
- Chapter 15, Metabolism: Basic concepts and design. pp. 409 – 430.
- Chapter 16, Glycolysis and gluconeogenesis. pp.
- Chapter 17, The citric acid cycle. (can skip section on the glyoxylate cycle)
- Chapter 18, Oxidative phosphorylation. pp. 501 – 503, 506 – 522, 527 – 532.
- Chapter 20, The Calvin cycle and the pentose phosphate pathway. pp. 577 – 585.
- Chapter 22, Fatty acid metabolism. pp. 617 – 626, 631 – 641.
- Chapter 23, Protein turnover and amino acid degradation, pp. 656 – 663, 666 – 672.
- Chapter 24, The biosynthesis of amino acids. pp. 683 – 693, 700 – 705.
- Chapter 25. Nucleotide biosynthesis. pp. 709 – 723.
- Chapter 26. The biosynthesis of membrane lipids and steroids. pp. 732 – 742.
- Chapter 27. The integration of metabolism. pp. 760 – 770.
Section and page numbers refer to the 6th edition.